Journal: Applied and Environmental Microbiology

Article Title: Site-Directed Mutations in the Lanthipeptide Mutacin 1140

doi: 10.1128/AEM.00704-13

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: THY broth and THY agar media were used for antimicrobial activity assays. table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) b Source or reference(s) Strains E. coli DH5α F − φ80lacZΔM15 Δ( lacZYA-argF )U169 recA1 endA1 hsdR17 (rk−, mk+) phoA supE44 λ-thi-1 gyrA96 relA1 tonA Invitrogen M. luteus ATCC 10240 Antibiotic indicator strain ATCC S. aureus ATCC 25923 ATCC S. pneumoniae ATCC 27336 ATCC C. difficile UK1 41 , 42 S. mutans UA159 43 S. mutans ATCC 55676 ATCC JH1140 Wild-type strain 31 JH1140 Phe1Gly mutA ::Phe1Gly site-directed derivative This study JH1140 Phe1Ser mutA ::Phe1Ser site-directed derivative This study JH1140 Phe1Thr mutA ::Phe1Thr site-directed derivative This study JH1140 Phe1Ile mutA ::Phe1Ile site-directed derivative This study JH1140 Phe1Trp mutA ::Phe1Trp site-directed derivative This study JH1140 Trp4Ala mutA ::Trp4Ala site-directed derivative This study JH1140 Trp4insAla mutA ::Trp4insAla site-directed derivative This study JH1140 ΔTrp4 mutA ::ΔTrp4 site-directed derivative This study JH1140 DHA5Ala mutA ::Dha5Ala site-directed derivative This study JH1140 Ala s 7insAla mutA ::Ala s 7insAla site-directed derivative This study JH1140 Arg13Asp mutA ::Arg13Asp site-directed derivative This study JH1140 Phe17insAla mutA ::Phe17insAla site-directed derivative This study JH1140 Asn18Ala mutA ::Asn18Ala site-directed derivative This study JH1140 Trp4Ala-Arg13Asp mutA ::Trp4Ala Arg13Asp site-directed derivative This study JH1140 mut a mutA ::heterodiploid derivatives; Erm r This study Plasmids TOPO-TA Cloning vector; 3′ T overhangs; lacZ for blue/white screening; Kan r , Amp r Invitrogen pVA891 Suicide vector; Erm r 27 pVA891mutA Suicide vector; Erm r ; cloned mutA containing approx 500 bp of flanking DNA Oragenics Inc. Open in a separate window a Site-directed heterodiploid mutants of S. mutans containing two copies of mutA and pVA891 suicide vector. b Erm r , erythromycin resistant; Kan r , kanamycin resistant; Amp r , ampicillin resistant.

Techniques: Plasmid Preparation, Cloning, Clone Assay

Journal: The Journal of Infectious Diseases

Article Title: The Global Regulon sarA Regulates β-Lactam Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus In Vitro and in Endovascular Infections

doi: 10.1093/infdis/jiw386

Figure Lengend Snippet: Staphylococcus aureus Strains and Plasmids Used in This Study

Article Snippet: The S. aureus strains were routinely grown in tryptic soy broth (TSB) or TSB agar plates. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Strain or Plasmid Relevant Characteristic(s) Source or Reference(s) Strain 300-169 MRSA ( agr-I , SCC mec IV, and CC45) [ 12 , 16 ] 300-169 Δ sarA 300-169 sarA ::kan [ 12 ] 324-136 MRSA ( agr-I , SCC mec IV, and CC45) [ 12 , 16 ] 324-136 Δ sarA 324-136 sarA ::Tn917LTV1 [ 12 ] MW2 MRSA (SCC mec IV), USA400 [ 12 , 13 ] ALC5415 MW2 sarA ::kan [ 12 ] JE2 MRSA (SCC mec IV), LAC, USA300 [ 17 ] JE2 Δ sarA JE2 sarA ::kan with ALC6185 [ 12 ] JE2 Δ sarA /p sarA JE2 Δ sarA complemented with pALC1215 This study JE2 Δ sarA /p mecA JE2 Δ sarA complemented with pALC6185 This study JE2 Δ mecA Transposon mutant with insertion in S. aureus USA300_0032 NTML JE2 Δ sigB Transposon mutant with insertion in S. aureus USA300_2022 NTML S. aureus ATCC43300 MRSA ATCC S. aureus ATCC25923 MSSA ATCC Plasmid pALC6185 pEPSA5:: mecA Cm r [ 21 ] pALC1215 pSPT181:: sarA Tet r [ 22 ] Open in a separate window Abbreviations: ATCC, American Type Culture Collection; MRSA, methicillin-resistant S. aureus ; MSSA, methicillin-susceptible S. aureus ; NTML, Nebraska Transposon Mutant Library; SCC mec , staphylococcal chromosome cassette mec .

Techniques: Plasmid Preparation, Mutagenesis